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International Journal of Surgical Pathology
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*Prostate Cancer
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A Novel Method of Obtaining Prostate Tissue for Gene Expression Profiling

Prasanna Sooriakumaran, BMedSci(Hons), BMBS(Hons), MRCS, PhD, PgCert(MedLaw)

Department of Urology, Royal Surrey County Hospital, sooriakumaran@ gmail.com

Alastair Henderson, MBBS, MRCS, PhD

Department of Urology, Royal Surrey County Hospital

Philippa Denham, MBBS, FRCPath

Department of Pathology, Frimley Park Hospital Surrey, United Kingdom

Stephen EM. Langley, BSc, MBBS, MS, FRCSUrol

Department of Urology, Royal Surrey County Hospital

Gene expression profiling by DNA microarray analysis is a technique with great promise in cancer biology. The multifocality and heterogeneity of many prostate cancers makes the collection of adequate biological samples for such profiling particularly challenging. Current methods, such as laser capture microdissection, are not widely available and can have significant limitations. In this article, a novel method of prostatic sampling, which does not affect the histopathological assessment of the surgical specimen and provides adequate RNA yield for microarray analysis is described. This method is simple, inexpensive, easily reproducible, and has been validated as having >95% sensitivity and 99% specificity for histological prediction of tissue obtained. This method can be adopted by other investigators to perform DNA microarray analysis on prostate tumors.

Key Words: prostatic sampling • gene expression • DNA microarray

This version was published on June 1, 2009

International Journal of Surgical Pathology, Vol. 17, No. 3, 238-243 (2009)
DOI: 10.1177/1066896908316902
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