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Isolated Tumor Cells and Circulating CK20 mRNA in pN0 Colorectal Cancer Patients

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong, cesar01@.netvigator.com

Mong-Tong Cheung, FHKAM

Department of Surgery, Queen Elizabeth Hospital

Brigette Buig-Yue Ma, FHKAM

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong

Edwin Pun Hui, FHKAM

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong

Alex Chak-Lam Chan, FRCP

Department of Pathology, Queen Elizabeth Hospital Hong Kong Special Administrative Region, China

Chi-Kin Chan, FHKAM

Department of Surgery, Queen Elizabeth Hospital

King-Chung Lee, FRCP

Department of Pathology, Queen Elizabeth Hospital Hong Kong Special Administrative Region, China

Wah Cheuk, FRCP

Department of Pathology, Queen Elizabeth Hospital Hong Kong Special Administrative Region, China

Money Yan-Yee Lam, MPhil

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong

Manson Chi-Keung Wong, BSc

Department of Pathology, Queen Elizabeth Hospital Hong Kong Special Administrative Region, China

Charles Ming-Lok Chan, MPhil

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong

John Kwok-Cheung Chan, FRCP

Department of Pathology, Queen Elizabeth Hospital Hong Kong Special Administrative Region, China

Anthony Tak-Cheung Chan, MD

State Key Laboratory in Oncology in South China, Sir Y K Pao Centre for Cancer, Department of Clinical Oncology, Hong Kong Cancer Institute and Prince of Wales Hospital, The Chinese University of Hong Kong

Micrometastases in lymph nodes and blood may provide important prognostic information. In this study, cytokeratin 20 (CK20) positive cells in lymph nodes and circulating CK20 mRNA were studied using 57 paraffin-embedded lymph node specimens and blood from 24 patients with pN0 colorectal cancer (CRC), respectively. Results showed that 29 out of 56 (52%) lymph node specimens had CK20-positive cells (range: 1-35). Follow-up of the patients for 12 months indicated that 4 patients (7%) had CRC metastases to liver, lung, and bone. In addition, 8 out of 24 (33%) samples had at least 2-fold circulating CK20 mRNA expression higher than the pooled normal sample. This study provides evidence that CK20-positive cells were found in the lymph nodes and differentially expressed circulating CK20 mRNA was also detected in the blood from patients with pN0 CRC. Long-term follow-up is necessary to study their prognostic use in patients with non-metastatic CRC.

Key Words: CK20 • lymph nodes • CK20 mRNA • pN0 • colorectal cancer patients

This version was published on April 1, 2008

International Journal of Surgical Pathology, Vol. 16, No. 2, 119-126 (2008)
DOI: 10.1177/1066896907311901


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